Journal: Polymers

Article Title: In Vitro Investigation of Thiol-Functionalized Cellulose Nanofibrils as a Chronic Wound Environment Modulator

doi: 10.3390/polym13020249

Figure Lengend Snippet: Cell metabolic activity of human dermal fibroblasts after 24 h exposure to cys-CNF and c-CNF suspensions. Data are expressed as percentage of the negative control (non-exposed cells) and presented as mean ± standard error of the mean of at least three independent experiments. The positive control represents cells exposed to 5% DMSO. Significant results compared with the negative control are marked with * ( p < 0.05).

Article Snippet: Adult human dermal fibroblasts (hDF) (primary cells from the European Collection of Authenticated Cell Cultures (ECACC, Salisbury, England) were cultured in DMEM-F12 medium supplemented with 10% v/v fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin.

Techniques: Activity Assay, Negative Control, Positive Control

Journal: Polymers

Article Title: In Vitro Investigation of Thiol-Functionalized Cellulose Nanofibrils as a Chronic Wound Environment Modulator

doi: 10.3390/polym13020249

Figure Lengend Snippet: Representative images of calcein-AM stained human dermal fibroblasts after 24 h exposure to cys-CNF and c-CNF suspensions. The negative and positive controls were non-exposed cells and cells exposed to 5% DMSO, respectively. Scale bar represents 100 μm.

Article Snippet: Adult human dermal fibroblasts (hDF) (primary cells from the European Collection of Authenticated Cell Cultures (ECACC, Salisbury, England) were cultured in DMEM-F12 medium supplemented with 10% v/v fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin.

Techniques: Staining

Journal: Molecular Biology of the Cell

Article Title: Ventral stress fibers induce plasma membrane deformation in human fibroblasts

doi: 10.1091/mbc.E21-03-0096

Figure Lengend Snippet: Sources for materials.

Article Snippet: Neonatal HDFs , Lonza (CC-2509) , 0000490825, 0000490827, 0000493458.

Techniques: Cell Culture, Recombinant, Immunofluorescence, Membrane, Staining, Plasmid Preparation, Cloning, Electron Microscopy, Western Blot

Journal: PLoS ONE

Article Title: Angiopoietin-Like 7 Is an Anti-Angiogenic Protein Required to Prevent Vascularization of the Cornea

doi: 10.1371/journal.pone.0116838

Figure Lengend Snippet: The areas of tube formation by HUVECs under the normal serum-free medium conditions were 6.81 ± 1.07% in co-culture with the human corneal keratocytes (HCKs, A) and 10.37 ± 2.52% in co-culture with the human dermal fibroblasts (HDFs, p < 0.0001, B and C). N = 12 per condition.

Article Snippet: Primary HDFs and HUVECs were purchased from Lonza (Allendale, NJ, USA).

Techniques: Co-Culture Assay

Journal: International Journal of Molecular and Cellular Medicine

Article Title: Osteogenic potential of human dental pulp stem cells, and human dermal fibroblasts exposed hydroxyapatite nanoparticles: A comparative in vitro study

doi: 10.22088/IJMCM.BUMS.14.2.656

Figure Lengend Snippet: Viability assay for hDPSCs and hDFs incubated with HA-NPs on 24 and 72h.

Article Snippet: Previous research covered the characterization and isolation of hDPSCs (Additional file) ( ). hDFs were acquired from the Iranian cell bank of the Pasteur Institute (NCBI Code: C646).

Techniques: Viability Assay, Incubation

Journal: International Journal of Molecular and Cellular Medicine

Article Title: Osteogenic potential of human dental pulp stem cells, and human dermal fibroblasts exposed hydroxyapatite nanoparticles: A comparative in vitro study

doi: 10.22088/IJMCM.BUMS.14.2.656

Figure Lengend Snippet: Microscopic views of Alizarin Red S staining of cultured hDPSCs and hDFs after 14 days in control medium (A&D), osteogenic medium (OM; B&E) and HA-NP medium (C&F). (G) A bar chart indicating the quantitative findings of differentiated hDPSCs and hDFs stained with alizarin red S after 14 days of culture. **p<0.01; ***p<0.001 for each group compared to the control group. differences between groups. #p<0.05; ###p<0.001. Abbreviations: hDPSC, human dental pulp stem cells; hDF, human dermal fibroblasts; OM, osteogenic medium, NP; nanoparticle.

Article Snippet: Previous research covered the characterization and isolation of hDPSCs (Additional file) ( ). hDFs were acquired from the Iranian cell bank of the Pasteur Institute (NCBI Code: C646).

Techniques: Staining, Cell Culture, Control

Journal: International Journal of Molecular and Cellular Medicine

Article Title: Osteogenic potential of human dental pulp stem cells, and human dermal fibroblasts exposed hydroxyapatite nanoparticles: A comparative in vitro study

doi: 10.22088/IJMCM.BUMS.14.2.656

Figure Lengend Snippet: ALP activity of hDPSCs, and hDFs cultured for 14 days in OM and NP medium. *p<0.05 for each group compared to the control group. ; Differences between groups. Abbreviations: hDPSCs, human dental pulp stem cells; hDF, human dermal fibroblasts; OM, osteogenic medium; NP, nanoparticle; ns, nonsignificant.

Article Snippet: Previous research covered the characterization and isolation of hDPSCs (Additional file) ( ). hDFs were acquired from the Iranian cell bank of the Pasteur Institute (NCBI Code: C646).

Techniques: Activity Assay, Cell Culture, Control

Journal: International Journal of Molecular and Cellular Medicine

Article Title: Osteogenic potential of human dental pulp stem cells, and human dermal fibroblasts exposed hydroxyapatite nanoparticles: A comparative in vitro study

doi: 10.22088/IJMCM.BUMS.14.2.656

Figure Lengend Snippet: Gene expression levels of (A) BMP-2, (B) RUNX2, (C) IL-6, and (D) VEGF-A in cultured hDFs and hDPSCs for 14 days in the presence of OM and NP. *p<0.05, **p<0.01; ***p<0.001 for each group compared to the control group. ; differences between groups. Abbreviations: hDPSC, human dental pulp stem cells; hDF, human dermal fibroblasts; OM, osteogenic medium; NP, nanoparticle; ns, nonsignificant.

Article Snippet: Previous research covered the characterization and isolation of hDPSCs (Additional file) ( ). hDFs were acquired from the Iranian cell bank of the Pasteur Institute (NCBI Code: C646).

Techniques: Gene Expression, Cell Culture, Control